Thy1 gcamp6f

Author: opos

August undefined, 2024

Webb15 mars 2024 · To generate GCaMP6 transgenic mice, we utilized the previously described GCaMP6 fast and slow cDNA constructs (referred as GCaMP6F and GCaMP6S, … WebbStock No: 025393 Protocol 28487: Standard PCR Assay - Tg (Thy1-GCaMP) Version 2. 2 Notes This assay will NOT distinguish hemizygous from homozygous transgenic animals. The genotyping protocol (s) …

Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo

WebbGCaMP6f expression allowed for population-based calcium recording from cortical excitatory neurons, in addition to concurrently acquired hemodynamics. All mice were fitted with a transparent Plexiglas window to facilitate imaging. Webb29 sep. 2014 · We use the Thy1-GCaMP6f mouse line from Jackson and it works beautifully. Reply. Jason says: February 3, 2024 at 12:03 pm. Alberto – which Thy1-6f line have you used? (Tg(Thy1-GCaMP6f)GP5.17Dkim by chance?). Reply. Tobias Rose says: April 14, 2016 at 12:08 pm. dragons eye pics

GENIE Reagents Janelia Research Campus

WebbIn vivo calcium imaging reveals that cortisol treatment reduces the number of place cells in Thy1-GCaMP6f transgenic mice Frontiers in Neuroscience Neuropharmacology March 1, 2024 ... Webb18 nov. 2024 · Mice (Tg-Thy1-GCaMP6f-GP5.17DKim/J, JAX 025393, The Jackson Laboratory) were initially anesthetized with 2.5% isoflurane in an induction chamber, and anesthesia was maintained with 1%-1.5% isoflurane during surgical procedures. Body temperature was maintained at ∼37°C using a temperature-controlled heating pad. WebbMesoscopic imaging of Ca2+ fluorescence through transparent polymer skulls implanted on transgenic Thy1-GCaMP6f mice reveals widespread activation of the cerebral cortex during locomotion, including not just primary motor and somatosensory regions but more »... premotor, auditory, retrosplenial, and visual cortices. emma casey tavistock

Strain Report - Rat Genome Database - Medical College of Wisconsin

THY1 Gene - GeneCards THY1 Protein THY1 Antibody

WebbThy1 Gcamp6f, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebbHere we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 … emma carter browningWebb6 juli 2024 · Mesoscopic imaging of Ca2+ fluorescence through transparent polymer skulls implanted on transgenic Thy1-GCaMP6f mice reveals widespread activation of the … emma cartoons for kids

"WebbSignificance: In vivo imaging and electrophysiology are powerful tools to explore neuronal function that each offer unique complementary information with advantages and limitations. Capturing both data types from the same neural population in the " - Thy1 gcamp6f

Thy1 gcamp6f

Functional connectivity structure of cortical calcium dynamics in ...

WebbThe GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics. 025393 - GP5.17 Strain Details If you need help with your … Webb13 sep. 2024 · (E and F) Setup for two-photon laser scanning microscopy (TPLSM) used for live imaging of mitochondria in individual RGC axons from Thy1-CFP-MitoS mice. (G and H) Representative kymographs of TPLSM-recorded mitochondrial movement show anterograde (magenta) and retrograde (green) transport in sham and ocular …

Did you know?

Webb17 juni 2024 · GCaMP6f, jGCaMP7f, jGCaMP7b ... Dana, H. et al. Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population … WebbAdult Thy1 Gcamp6f Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Webb24 sep. 2014 · Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions … WebbIn a Thy1::GCaMP6f transgenic mouse, PSAM 4-GlyR was expressed in hippocampal pyramidal cells by injection of rAAV1-CamkII::PSAM 4-GlyR–IRES–EGFP . The field of view contained distinct domains of neurons densely and sparsely transduced with PSAM 4-GlyR–IRES–EGFP .

Webb11 okt. 2024 · Calcium imaging is commonly used to measure the neural activity of large groups of neurons in mice. Genetically encoded calcium indicators (GECIs) can be delivered for this purpose using non-invasive genetic methods. Compared to viral gene transfer, transgenic targeting of GECIs provides stable long-term expression and … Webb5 apr. 2024 · Qualitatively, imaging the cortex of a Thy1-GCaMP6f mouse 23, which expresses fluorescent reporters of calcium activity in excitatory neurons, with the mini …

WebbTo demonstrate the fundamental capacity for calcium imaging in deep regions of the rodent brain using FONT, we developed and validated here a GCaMP6f-expressing in situ mouse brain model intracardially perfused with artificial cerebrospinal fluid that overcomes the limitations of low penetration at visible wavelengths.

WebbGCaMP6f was excited at 920 nm (InSight DS Dual, Spectra-Physics), and images were acquired with an upright two-photon laser-scanning microscope (FV1000MPE, Olympus) using a water-immersion 10× objective lens (UMPLFLN 10XW; NA, … dragons fate wotrWebb5 nov. 2024 · We integrated an advanced CMOS image sensor into a popular open-source miniaturized microscope platform. MiniFAST is a fast and sensitive miniaturized microscope capable of 1080p video, 1.5 µm... dragons eye sealWebbStrain reports include a comprehensive description of function and biological process as well as disease, expression, regulation and phenotype information. dragons fire flight line haiti