Web2 aug. 2013 · DHHC2 and DHHC3 fused to Renilla luciferase or YFP retain PAT activity. HEK-293 cells were transfected with plasmids encoding DHHC2 (A) ... activity of the DHHC fusion proteins was confirmed by in vitro assays using an N-terminal fragment of N-myristoylated Lck as a substrate (Fig. 1, A and B, top panels). Web12 mei 2024 · No polarization of DHHC2 in the dhhc2::6HA gametocytes with only either XA or 22°C stimulation. Three replicates are performed, and values are means ± SD. No change in DHHC2 expression in zygotes of the dhhc2::6HA parasite-derived mutants with individual gene disruption of cdpk4, hap2, and dozi.
Molecular membrane biology.-2011年 第7a8期-外文期刊【掌桥科 …
WebHuman DHHC2 and yeast Pfa3 were assayed with their respective N-myristoylated substrates, Lck and Vac8. Human DHHC9/GCP16 and yeast Erf2/Erf4 were tested using … Web13 jan. 2012 · DHHC2 and DHHC3 showed similar levels of palmitate incorporation, ~2.5 times that of DHHC9/GCP16. 5 In vivo evidence suggests that DHHC2 and DHHC3 have broad protein substrate specificity . Both expressed well in insect cells infected with recombinant baculovirus and were purified to near homogeneity using nickel chelate and … jobs in kathu northern cape
DHHC2 is a protein S-acyltransferase for Lck - PubMed
Web1 feb. 2009 · To test Lck as a substrate for DHHC2, we used a model substrate consisting of the N-terminal myristoylation, palmitoylation motif (SH4 domain) and the SH3 and SH2 … Web1 feb. 2012 · DHHC2 was also found to be engaged in the regulation of SNAP25/23 palmitoylation dynamics at the plasma membrane ( Greaves et al., 2010 ). Moreover, some experiments have revealed that the nonreceptor tyrosine kinase Lck ( Jennings et al., 2009) and eNOS ( Fernandez-Hernando et al., 2006) were good substrates for DHHC2. … Web17 okt. 2024 · While cigarette smoking is the predominant form of tobacco consumption in the United States and other countries, consumption of smokeless tobacco products (STPs) is also common. 4 Existing US and Swedish epidemiological data indicate that consumption of STPs is less harmful compared with cigarette smoking and the health risks associated … jobs in kearsley farnworth