2024 Hygromycin b resistance cassette

Hygromycin b resistance cassette

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Web11 jan. 2024 · Using a method based on homologous recombination described previously 11, the target genes were deleted with a hygromycin B resistance cassette or the promoters of the target genes were replaced ... Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique Eco RI, Pst l, and Sac ll sites in the coding sequence of a hygromycin B phosphotransferase gene ( …

Split selectable markers Nature Communications

Web15 okt. 2005 · We describe here three new cassettes for PCR-mediated gene disruption that can be used in combination with commonly used fission yeast markers to make … WebName Vector Type Resistance Marker Bacterial Resistance Source Sequence Available; 7749 Bicistronic_ires_hygro: Retroviral: Hygromycin: Addgene: bRA66: Yeast Expression, CRISPR, Synthetic Biology paracord halsband hund anleitung

Zeocin treatment significantly elevated transformation efficiency of

Web21 apr. 2014 · The hygromycin B (Hyg R) resistance marker used in this study confers resistance by the activity of a specific a minoglycoside ph osphotransferase encoded … WebHygromycin B resistance as dominant selectable marker in yeast. Saccharomyces cerevisiae is normally sensitive to the drug hygromycin B; a hygromycin B concentration … WebCan anyone share a sequence of hygromycin resistance gene or any other antibiotic resistance gene (except mentioned above) that can be … paracord halsband maken

Split selectable markers Nature Communications

Golden Gate vectors for efficient gene fusion and gene deletion in ...

WebThe hygromycin-resistance cassette hygroR, containing hph gene under the control of N. crassa pCPC1 and A. nidulans tTrpC, was amplified by PCR using primer pair neoHY-8 … Web1 dec. 2003 · Therefore, the aim of this work was to design a more efficient gene knockout method for use in this yeast. To simplify the use of multiple selection markers, we have developed disruption cassettes containing the homologous ylURA3 and ylLEU2 genes, and the heterologous marker hph conferring resistance to hygromycin-B (Hyg) into the … paracord hardware wholesaleWebHygromycin B. Hygromycin B interacts with 16S rRNA in a manner different from that of paromomycin, streptomycin, or spectinomycin. 248 Hygromycin B binds close to the A site near the top of helix 44 of 16S rRNA, 191, 198, 228 with one ring ... Here, the marker corresponds to the hygromycin resistance cassette ... paracord halsbanden

"Web21 apr. 2014 · The hygromycin B (Hyg R) resistance marker used in this study confers resistance by the activity of a specific a minoglycoside ph osphotransferase encoded by … " - Hygromycin b resistance cassette

Hygromycin b resistance cassette

pSELECT-hygro : Dual expression cassette plasmid with hygromycin …

Web1 jan. 2010 · Gene-specific knockout cassettes should be designed to have 1-1.3 kb of gene-specific 5′ and 3′ flanks and a selectable marker (the hygromycin B phosphotransferase gene, hph, in our case). To ensure appropriate expression of the selectable marker, we use the A. nidulans promoter trpC ( 13 ). Web29 sep. 2024 · PDA plates supplemented with 20 μg/mL of hygromycin B resulted in complete growth inhibition of Lophiotrema sp. F6932 wild-type strain, an indication that hygromycin B resistance gene would be suited for used as a selectable marker for Lophiotrema sp. F6932 knockouts mutants (Supplementary Figure S4). Protoplast …

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WebHygromycin B. 41,42 Hygromycin B (Figure 8), produced by S. hygroscopicus, exhibits activities against both prokaryotic and eukaryotic cells. From: Comprehensive Medicinal … Web26 jan. 2024 · Primary transformants were crossed with the color spore mutant fus1-1 (S23442) and single-spore isolates (ΔSmlon2) with hygromycin resistance were selected . The absence of the Smlon2 gene and the integration of the hph -cassette at the desired locus was verified using primer pairs lon2-ko-v5f/tC1_0 (1400 bp), h3_0/lon2-ko-v3r …

Webhygromycin B 70-O-kinase or simply hygromycin phosphotrans-ferase [14]. The specific modification of hygromycin B is a phosphorylation at the 70-OH of the destomic acid ring [15]. Resistance to apramycin (AprR) is conferred by the 777 bp aac(3)IV aminoglycoside 3-N-acetyltransferase gene [13]. Specifically, the WebTransformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers …

Web2 feb. 2024 · Sequence of hygromycin resistance cassette for mutant generation. The cassette includes the FRT site (red), promoter site for hygromycin (green), and the hygromycin resistance gene (blue). (DOCX) Browse. Search. Explore more content. ppat.1009291.s004. docx (20.59 kB) File info Download file. WebExpression plasmid with hygromycin resistance gene. 20 µg . pseth-mcs. TDS MSDS +-$535.00 Add to favorite. pSELECT-hygro-LacZ Unit size Cat. code Docs Qty ... the two expression cassettes are oriented in convergent directions ... Hygromycin B Gold . Selective antibiotic for the hph gene ...

WebCpD14 knockout mutants were obtained through double homologous recombination with a construct carrying a hygromycin B resistance cassette interrupting the gene sequence. Around 1 kb upstream and downstream sequences of the CpD14 gene were used to induce homologous recombination.

WebHygR 345 .. 1373 = 1029 bp 342 amino acids = 38.1 kDa Product: aminoglycoside phosphotransferase from E. coli confers resistance to hygromycin HygR 345 .. 1373 = … paracord halsband setWebArticle Snippet: The hygromycin B resistance cassette was amplified through PCR from the pCB1004 vector (Carroll et al ., ) using the Gibson assembly primer couple GibFor … paracord handcuff key buckleWeb24 dec. 2024 · For gene deletion, we provide five different donor vectors for selection marker cassettes. These include standard cassettes for hygromycin B, nourseothricin and … paracord headphonesWeb8 mrt. 2024 · The hygromycin B resistance gene was detected in all tested transformants (Supplementary Fig. 3). Discussion In this study, we developed a high-efficiency N. oceanica transformation method in which the transformation efficiency was significantly elevated by at least one order of magnitude when the parental algal cells were pretreated with zeocin at … paracord handles for walking sticksWebWe have utilized the promoter cassettes to express the hygromycin-resistance gene either from the CaMV 35S or the CaMV 19S promoters, with both chimeric resistance … paracord headbandWeb31 okt. 2024 · By combining split points, we create 3- and 6-split Hygromycin resistance genes, ... by removing Puromycin resistance gene and inserting IRES-fluorescent genes downstream of the Gateway cassette. paracord hat band diyWeb24 dec. 2024 · Hygromycin B- and nourseothricin-resistant transformants with red ascospores were isolated, analyzed by PCR for correct integration of the deletion cassette, and crossed to wildtype to dispose of the NHEJ-deficiency and to recycle the nat1 marker. paracord hat band instructions