Human oct4
WebCreative Biolabs offers high-quality NeuroMab™ Anti-OCT4 Antibody, Clone N5583P to boost neuroscience research. Toggle navigation. Service . In Vitro Services. ... Synthetic peptide derived from an internal portion of human OCT4. [Uniprot: Q01860] Species Reactivity. Human. Clonality. Monoclonal. Host Species. Mouse. Isotype. IgG1. Clone ... WebLevels of OCT4 are down-regulated during differentiation and it has thus emerged as a useful marker of pluripotency in stem cells, as well as a marker for certain human malignant germ cell tumours. Expression of OCT4 together with other transcription factors has been used to reprogram somatic cells into iPS cells.
Human oct4
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Web31 jan. 2014 · Oct4 is a key transcription factor in stem cell research and cellular reprogramming. Since it has been shown that recombinant Oct4 fused to a cationic … Web2 apr. 2012 · Endogenous OCT4 protein was affinity-purified from human ES cells using five lysis methods (Materials and Methods and Fig. S1A). We included a range …
WebWestern blot - Human Oct4 peptide (ab20650) Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct-4 in human ES cell lysate. In mouse ES … Web5 okt. 2024 · The human OCT4 enhancer controlled pGreenFire Lenti-Reporter was from System Biosciences (Palo Alto, CA). Genomic DNA was extracted from bovine Wharton’s jelly cells using DNAzol (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol.
Web29 mrt. 2024 · Oct4 promoted proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in colon cancer cells by activating the SCF/c-Kit signaling pathway. Bu … Web1 jan. 2014 · To test this, the authors used human OCT4 purified from E. coli inclusion bodies and incubated it with CVI-5B cells and human BJ foreskin fibroblasts at a concentration of 100 nM. Immunostaining revealed that the OCT4 protein penetrated both cell types, suggesting that the unmodified OCT4 protein can be used as self-penetrating …
Web11 okt. 2007 · To approach this project, we began with an initial alignment of Oct4 with its alternative splice variants and its pseudogenes. This alignment served as a basis for an exact primer design. Initially, we clarified the sequence and organization of the functional human Oct4 gene, to allow comparison to the pseudogenes and alternatively spliced …
WebIn serum-free hESC culture medium, basic fibroblast growth factor (bFGF) is required to maintain expression of Oct4, a pluripotent gene. Unexpectedly, hESCs cultured on nanotopography could maintain Oct4 expression without bFGF supplementation. With bFGF supplementation, the HEX nanotopography maintained Oct4 expression whereas the … smallsh osu githubWeb4 apr. 2024 · INTRODUCTION. Mitochondria and their 16.5-kb circular genomes (mtDNA) undergo constant turnover in arrested primary oocytes for decades in humans (1, 2).Frequent DNA replication and lack of efficient DNA repair mechanism in the mitochondrion contribute to a mutation rate that is ∼10-fold higher than that of the … hilcorp fortune 500Web5 sep. 2014 · In humans, Oct4 mRNA is present throughout all stages from the unfertilized oocyte to the uncompacted morula [16-18]. These stages display a … hilcorp frcWeb25 apr. 2024 · While the genome-wide targets of OCT4 have been mapped in both mouse and human ESCs 32, 33, 34, 35, its physical interactome in hESCs has not to date been … smallshaw family crestWeb21 mrt. 2024 · OCT4 3 4 POU-Type Homeodomain-Containing DNA-Binding Protein 3 POU Domain Class 5, Transcription Factor 1 2 POU Domain Transcription Factor OCT4 3 … smallshaw ashton under lyneWeb14 jun. 2024 · Octamer‑binding transcription factor 4 (Oct4) has been identified as a novel transcription factor associated with tumorigenesis, acquisition and maintenance of cancer stem cell characteristics and poor prognosis in tumors. However, the role of Oct4 in tumorigenesis and progression of hepatocellular carcinoma (HCC) has not yet been fully … smallsh cs 344Web15 jun. 2024 · Recent KO experiments have also highlighted a different function of OCT4 in rodents vs. ungulates and humans. Oct4 KO mouse embryos develop to blastocyst, although hypoblast formation—explained in the next section—is impaired, but the absence of OCT4 protein in OCT4 KO morulae has been observed to impair blastocyst formation … hilcorp founder