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Ctll 2 proliferation assay

WebApplication CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. [ 1] [ 2] Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS). Subculture Routine Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. WebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa.

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WebApr 5, 2024 · measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL. Two independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-FreeTM Recombinant Human IL-2. %LRDFWLYLW\ WebFor example, Recombinant Human IL-2 can activate proliferation of IL-2 dependent cell lines, CTLL-2 and HT-2. The same lot of recombinant protein provides different ED 50 values depending on the cell line used for stimulation. In the CTLL-2 stimulation assay, the ED 50 is 0.26 ng/ml corresponding to a specific activity of 3.73 x10 6 units/mg. spanish word for rattle https://oahuhandyworks.com

Life-extended glycosylated IL-2 promotes Treg induction and suppression

Webfrom the Mo7e and CTLL-2 cyto-kine-dependent proliferation assay, PFC-1 exhibited an approx-imately 10-fold stronger potency than rhIL-15 in PBMC prolifera-tion, while 10 nM rhIL-15 led to a proliferation rate of 20.71% and 1 nM PFC-1 resulted in a mean value of 22.05% (Fig. 2B). The enhanced activity could be due to the higher activity of IL ... http://www.wrenshallab.com/uploads/5/6/3/8/56385051/mttctll2.pdf WebAsn77 of IL-15 undergoes deamidation with a t 1/2 of ~4 days at pH 7.4, 37°C, but the IL-15 (Asp77) product is equipotent in the CTLL-2 bioassay and <5% additional degradation occurred over 8 days. 27 28 Here, when IL-15 was kept at pH 7.4, 37°C, for 10 days, there was no significant change in EC 50 in cell-based IL-2Rβ/γc dimerization or ELISA … teatro thuiller

A universal reporter cell line for bioactivity evaluation of …

Category:CTLL-2 93042610 Sigma-Aldrich

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Ctll 2 proliferation assay

(PDF) Combined treatment of autoimmune MRL/MP-lpr/lpr mice …

WebOct 31, 2000 · In this study, alamarBlue was used to measure the proliferation of the IL-2-dependent cytotoxic T cell line, CTLL-2. The colorimetric change of alamarBlue at 570 nm compared to the reference wavelength, 600 nm, was proportional to the number of viable cells. The sensitivity of the IL-2 assay using ... proliferation assays. PDF; Standard … WebCellGenix® GMP rh IL-2 has an activity of 8 – 32 × 106 IU/mg. The activity of GMP rh IL-2 was measured in a cell proliferation assay using the IL-2-dependent cell line CTLL-2. It was calibrated against Proleukin®. You can find the batch specific activity on the certificate of analysis (CoA).

Ctll 2 proliferation assay

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WebCTLL-2 proliferation assay CTLL-2 cells were washed twice with prewarmed HBSS (Gibco, # 14175095) and then grown in the absence of IL-2 for 24 h in RPMI1640 (Gibco, … WebCTLL-2 is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon Interleukin-2 (IL-2) for growth and can be used to assay for IL-2. …

WebTitle: CTLL-2 Cell Proliferation Assay for Determination of Bioactivity of IL-15 . This procedure is made available through federal funds from the National Cancer Institute, NIH, under contract . UNCONTROLLED COPY FOR TRAINING AND REFERENCE PURPOSES ONLY . 7.5.2 Using a 50 -300 multiple channel pipettes, add the Starting Std01 or sample WebProliferation: IL-2 : CTLL-2 (ATCC TIB-214) 2: 24: 10: ≤175pg/mL: Proliferation: IL-3 : TF-1 (ATCC CRL-2003) 2: 48: 10: ≤0.6-0.15ng/mL: Proliferation: IL-4 : TF-1 (ATCC CRL-2003) 2: 48: 10: ... Cytotoxicity Assay: VEGF 121: HUVEC: 2: 72: 1000: ≤8-10ng/mL: Proliferation: Mouse Cytokine Bioassay Quick Guide Cytokine Indicator Cells Cell ...

WebApr 13, 2024 · of Recombinant Human IL-2 as measured in a cell proliferation assay CTLL 2 mouse cytotoxic T cells (orange, green, red, respectively). 6'6 3$*(5HFRPELQDQW+XPDQ,/ 3URWHLQ6'6 3$*( 1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver … WebJun 2, 2012 · A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the ability to …

WebThe mouse T-cell line CTLL-2 is known to be dependent on interleukin-2 (IL-2) for both growth and viability. ... Although only very limited proliferation was observed during the incubation as assessed by 3H-thymidine incorporation, the viability of the CTLL-2 cells on the immobilized IL-2 matrix was preserved. Cells incubated on the immobilized ...

WebWhen maintained in continuous culture, however, the overall sensitivity of this cell line is highly variable. To overcome this problem, large batches of CTLL-2 were grown, frozen and tested for their ability to detect low levels of IL-2 (0.01 IU) in dose response assays. Batches meeting this criterion were stored in liquid nitrogen until required. teatro the globeWebTitle: CTLL-2 Proliferation Assay for Determination of Bioactivity of Recombinant Human Interleukin 2 (rhIL-2) UNCONTROLLED COPY FOR TRAINING AND REFERENCE … spanish word for requestWebNov 29, 2013 · Most bioassays measured the proliferative effect of IL-2 on the murine cytotoxic T cell-line, CTLL-2 or the HT-2 cell-line but employed different readouts for assessing the proliferation (Gillis et al., 1978, Gieni et al., 1995). In one laboratory, however, the human T cell-line, KIT 225 was used (Hori et al., 1987). teatro tlaxcala