WebJan 5, 1999 · Assignee: Clontech Laboratories, Inc. Inventors: Steve Kain, Xianqiang Li One step RT-PCR methods, enzyme mixes and kits for use in practicing the same ... to the C-terminal end of an enhanced variant of GFP (EGFP). Fluorescence intensity of the fusion protein in transfected cells is similar to that of EGFP, but the fusion protein, unlike EGFP ... WebA commercial form of this recombineering technology called In-Fusion (®) that facilitates high-throughput directional cloning of PCR products has been commercialized by Clontech. To effect the in vitro cloning reaction, PCR products are prepared using primers that add 16-18 bp of sequence to each end of the PCR amplicon that are homologous to ...
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WebOct 20, 2016 · The cornerstone of In-Fusion cloning technology is Clontech’s proprietary In-Fusion Enzyme, which fuses DNA fragments e.g. PCR-generated sequences and … WebAug 28, 2014 · Conclusion. Our In-Fusion HD Cloning Plus system can be used for both cloning and mutagenesis, eliminating the need for a separate kit for mutagenesis. It is a fast protocol that combines the ... offices at central world
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WebNov 6, 2024 · Takara Bio USA, Inc., manufactures and distributes products under the Takara™, Clontech®, and Cellartis® brands. These products include kits, reagents, and instruments for life sciences research applications, including NGS, PCR, gene delivery, genome editing, stem cell research, cloning, nucleic acid and protein purification, and … Webneria™ guard is an all-in-one soft infusion set with an integrated one-touch inserter. With a unique, automatic retracting needle, neria™ guard can help to minimise complications … WebClontech Laboratories, Inc. www.clontech.com Protocol No. PT4065-2 2 Version No. PR9Z3432 In-Fusion™ Advantage PCR Cloning Kit Protocol-at-a-Glance Protocol II. In-Fusion PCR Cloning Procedure w/Spin-Column Purification If a non-specific background smear or multiple bands are visible on your gel, isolate your target fragment by my d link camera access