WebVitamin D 1.25 - Dihydroxy Assays for Vitamins and Metabolic Function Utilization Guidelines In accordance with CMS Ruling 95-1 (V), utilization of these services should be consistent with locally acceptable standards of practice. Medicare recognizes certain tests may exceed the stated frequencies. Should a denial occur, additional ... WebNo matter which assay you use, the most important vitamin D measurement value is the final total value, since it represents the total amount of 25-hydroxy-vitamin D (both D 2 and D 3) in the blood. This ensures patients have the most accurate result regardless of level and whether or not they are supplemented over-the-counter or by prescription.

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