Brca2 shrna
WebApr 29, 2024 · The shRNA sequences targeting BRCA2 (sh-BRCA2-UTR: 5′-CGC TTA ACC TTT CCA GTT TAT-3′, sh-BRCA2-CDS: 5′-AGCTTA CCTTGAGGGTTATTT-3′) were cloned into lentiviral vector pLKO.1. … WebMar 29, 2024 · BRCA2: A gene that normally acts to restrain the growth of cells in the breast and ovary but which, when mutated, may predispose to breast cancer and to ovarian …
Brca2 shrna
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WebApr 28, 2014 · Breast cancer susceptibility genes 1 and 2 (BRCA1/2) are known as tumor suppressor genes playing critical roles in DNA repair, cell cycle checkpoint control, and in maintenance of the genomic stability. Both BRCA1 and BRCA2 suppress the cell cycle progression [ 6 – 8 ]. WebApr 6, 2024 · Breast cancer type 2 susceptibility (BRCA2) protein is crucial for initiating DNA damage repair after chemotherapy with DNA interstrand crosslinking agents or X-ray irradiation, which induces DNA double-strand breaks. BRCA2 contains a C-terminal RAD51-binding domain (CTRBD) that interacts with RAD51 …
WebMar 4, 2005 · We also procured two siRNAs designed from two different loci in the BRCA2 ORF from Qiagen. We used those anti-BRCA2 siRNAs in HMEC and BT549 cells. Third, we designed shRNA genes from those siRNAs and cloned them in pSUPER to express in vivo the anti-BRCA2 shRNA in transfected breast cells. WebAug 13, 2024 · Germline mutations in BRCA1 or BRCA2 tumour suppressor genes predispose individuals to breast, ovarian, prostate and other types of cancer. …
WebSuitable for use as control antibody for BRCA2 siRNA (h): sc-29825, BRCA2 shRNA Plasmid (h): sc-29825-SH and BRCA2 shRNA (h) Lentiviral Particles: sc-29825-V. Molecular Weight of BRCA2: 390 kDa. Positive Controls: MCF7 whole cell lysate: sc-2206, HeLa whole cell lysate: sc-2200 or PL-45 whole cell lysate. RECOMMENDED … WebSep 15, 2024 · The H1299 cells used in this study carry a doxycycline (DOX)-inducible short hairpin RNA (shRNA) against BRCA2 ( Zimmer et al., 2016 ), which enables the effective inhibition of BRCA2 expression after 48 h of DOX treatment ( Figure S1 A). Download : Download high-res image (1MB) Download : Download full-size image Figure 1.
WebFor induction of BRCA2 shRNA, 2 µg/mL DOX (Sigma-Aldrich, D9891) was added to growth medium for 3 days. Extracted molecule total RNA Extraction protocol Cells were collected for RNA extraction using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s guidelines.
WebBRCA2 Antibodies Santa Cruz Biotechnology, Inc. offers a broad range of BRCA2 antibodies. Select BRCA2 antibodies from monoclonal antibodies listed below. View detailed BRCA2 antibody specifications by linking to the specific product blocks. hisense uk opinionWebApr 6, 2024 · DPP8/9 inhibition induces either pyroptotic or apoptotic cell death in hematological malignancies. We previously reported that treatment with the DPP8/9 inhibitor 1G244 resulted in apoptotic cell death in myeloma, and our current study further evaluates the mechanism of action of 1G244 in different blood cancer cell lines. Specifically, 1G244 … hisense u8h malaysiaWebJun 17, 2024 · BRCA2 and QSER1 shRNA constructs were cloned into the pLKO.1 vector (Addgene). The Non-targeting shRNA construct (SHC002) was purchased from Sigma. Lentiviral particle preparation and infection were performed as described above. 24 hrs after infection, the ES cells were subjected to selection with 2 ug/mL of puromycin for an … hisense u7hqtukWeb目的探讨MCM7基因沉默介导AKT信号通路参与人皮肤黑色素瘤细胞的增殖及凋亡。方法构建MCM7基因和沉默MCM7基因表达的慢病毒RNA(LV-shRNA-MCM7)的表达载体;将A375细胞分为Control组、Emptyvector转染组(空载质粒转染组)、siRNA(LV-shRNA-MCM7)转染组 … hisense usa supportWebThermo Fisher h1299 carrying dox inducible brca2 shrna cells H1299 Carrying Dox Inducible Brca2 Shrna Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more hisense usa hqWeb在众多种类的转录因子中,干扰素调节因子是一类非常重要的成员。它被发现的最早的功能就是能够调节干扰素的表达。所有的干扰素调节因子家族成员都能够调节干扰素及干扰素相关基因的作用。另外,它在免疫应答、抗病毒、细胞生长调节以及细胞凋亡中发挥着必不可少的作用。迄今为止,其实 ... hisense u8hqtukWebMar 21, 2024 · BRCA2 (BRCA2 DNA Repair Associated) is a Protein Coding gene. Diseases associated with BRCA2 include Fanconi Anemia, Complementation Group D1 and Breast Cancer . Among its related pathways are Resolution of D-loop Structures through Holliday Junction Intermediates and Homologous DNA Pairing and Strand Exchange . hisense uhd