2024 Bodipy fl pepstatin a

Bodipy fl pepstatin a

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WebProduct Description Pepstatin A, BODIPY FL conjugate is a green-fluorescent probe for investigation of cathepsin D tracking in cells. Pepstatin A selectively binds to cathepsin D at pH 4.5. Specification Appearance Solid Powder Synonyms Pepstatin A, EverFluor FL Conjugate Reference Reading Customer reviews and Q&As Write a review Ask a question WebIn vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC 50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a fluorescence …

BODIPY FL - an overview ScienceDirect Topics

WebFor lysosomal pH, Cathepsin D assay or Cathepsin L assay, 1 µM LysoSensor Green (Invitrogen, L7535), 1 µM BODIPY FL-pepstatin A (Invitrogen, P12271) or 20 μl Magic Red (ImmunoChemistry Technologies, 941) were incubated with hepatocytes at 37°C for 30 min. For F-actin staining, 100 nM SiR-Actin (Cytoskeleton Inc, CY-SC001) were incubated in ... Web(A) Representative images of WT and sAC KO MEFs stained with BODIPY-FL–Pepstatin A. (B) BODIPY-FL–Pepstatin A intensity was quantified using MetaMorph in multiple cells from three independent ... cvs guilford rd columbia md

BDP FL carboxylic acid - Lumiprobe

WebBodipy Fl Pepstatin A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebMar 28, 2014 · The BODIPY FL-pepstatin A signal was excited at 488 nm using the microscope’s Argon laser; emission was read at 525 nm. Image capture and analysis were performed using Nikon Elements Advanced Research Software package 3.2. WebJan 9, 2024 · BODIPY-FL-pepstatin A for active cathepsin D staining was obtained from Thermo Fisher Scientific (P12271). The experiments were conducted in accordance with the protocol provided by the manufacturer. Briefly, the sciatic nerves from uninjured or injured mice were harvested, and then connective tissues were detached in PBS. cvs guilderland new york

Lysosomal adaptation: How cells respond to lysosomotropic

Pepstatin A, BODIPY FL Conjugate - BOC Sciences

WebMar 16, 2000 · The BODIPY FL fluorophore is very photostable and pH-insensitive, two important properties for both microscopic studies and enzymatic assay [16]. The … cheapest place to buy glock magazinesWebBODIPY™ FL vancomycin, which contains a single BODIPY™ dye per vancoymycin molecule, is a green-fluorescent analog of this important antibiotic, which is active against gram-positive bacteria, including entereococci.Although its biological activity has not been reported, it may be useful for detecting vancomycin binding sites and transport, for … cheapest place to buy giants tickets

"WebFig. 9. Saturation binding analysis for BODIPY FL GTP γ S binding to G αo in a 96-well plate format. G αo (20 n M) was incubated with the indicated concentrations of BODIPY FL … " - Bodipy fl pepstatin a

Bodipy fl pepstatin a

WebApr 25, 2011 · For BODIPY FL pepstatin A staining, cells were labeled with 1 μM BODIPY FL pepstatin A for 30 min, and then the probe-containing medium was replaced with fresh medium. Electron Microscopy. Cells were fixed in 3% glutaraldehyde in 0.1 M Mops buffer (pH 7.0) for 8 h at room temperature, then 3% glutaraldehyde/1% paraformaldehyde in … WebMay 6, 2024 · Using a new robust lysosomal pH biosensor (FIRE-pHLy), we developed a cell-based fluorescence assay for high-throughput screening (HTS) and applied it to differentiated SH-SY5Y neuroblastoma cells.

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WebBorondipyrromethene (BODIPY) dyes are bright and photostable fluorophores. BDP FL is a dye for fluorescein (FAM) channel. This is a free unactivated carboxylic acid derivative of … WebProduct Overview. Recommendations. Documents. For the study of cathepsin D trafficking in cells by fluorescence microscopy, try our BODIPY FL pepstatin A. This green … TC-FlAsH II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-b… For the study of cathepsin D trafficking in cells by fluorescence microscopy, try o… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes Support How can we help? Choose an option. Contact Us

WebSecond, we tested the specificity of BODIPY-FL-pepstatin A for detecting active cathepsin D by treating cortical neurons at DIV14 for 24 h with pepstatin A, an inhibitor of aspartic proteases,... Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆

WebJul 1, 2015 · To label active cathepsin D in mature lysosomes, live adult MNs or DRG neurons were incubated with 1 µM Bodipy-FL-pepstatin A in culture medium for 1 hr at 37°C. An active form of cathepsin B was labeled by the cresyl violet fluorogenic substrate CV-(Arg-Arg)2 (Magic Red). WebDec 28, 2024 · Lipid peroxidation was measured by staining using C11 BODIPY, and immunofluorescence images were taken using a laser-scanning confocal microscope (LSCM). ... glycerol (2%). EDTA (1 mM), DTT (1 mM), aprotinin (1 μg/ml), leupeptin (1 μg/ml), pepstatin (1 μg/ml), PMSF (1 ... levels was measured by counting the filipin …

WebMar 16, 2000 · In vitro, BODIPY FL–pepstatin A inhibits cathepsin D activity with an IC 50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a …

WebOther suitable methodologies rely on the use of fluorescently labelled inhibitors of lysosomal hydrolases such as Bodipy FL-Pepstatin A (Invitrogen, P12271), a … cheapest place to buy glasses near meWebInvitrogen™ Pepstatin A, BODIPY™ FL Conjugate For the study of cathepsin D trafficking in cells by fluorescence microscopy Brand: Invitrogen™ P12271 Additional Details : … cvs guilford collegeWebFor the study of cathepsin D trafficking in cells by fluorescence microscopy, try our BODIPY FL pepstatin A. This green-fluorescent probe, which inhibits cathepsin D in vitro (IC50 50 nM), targets cathepsin D within lysosomes of live cells. ... 对于通过荧光显微镜检查对细胞蛋白酶 D 运输进行的研究，请尝试使用我们 ... cvs gulf breeze proper hoursWebBODIPY FL Coumarin Cy3 Cy5 Fluorescein (FITC) Oregon Green Pacific Blue Pacific Green Pacific Orange PE-Cyanine7 PerCP-Cyanine5.5 Tetramethylrhodamine (TRITC) … cvs gulf breeze highwayWebMar 1, 2006 · In their study, BODIPY FL-pepstatin A inhibited the CD activity, with an IC 50 of 10 nM in vitro, and stained lysosomes, where it was co-localized with CD, in a murine macrophage-like cell line (J774A.1) within 30 min. From this, it is thought that 120 µM pepstatin A completely inhibits the CD activity in osteoclasts. These results indicate ... cvs gulfport ms hoursWebMar 16, 2000 · In vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a … cvs gulf breeze proper pharmacyWebMar 16, 2024 · High content LysoTracker ® Red (LTR), LysoSensor Green, Magic Red™ cathepsin B, Bodipy FL-pepstatin A for cathepsin D and phospholipid staining. ARPE-19 cells were seeded in 96-well plates at 5000 cells/well and allowed to attach overnight. The final DMSO concentration was 0.5% in vehicle control wells and compound treated wells. cheapest place to buy glitter